Olkowscy: ród – genealogia , historia rodzin. Olki-Kurzaątki, Niesułowo, ziemia różańska
Day: February 14, 2017
Biochemical Pharmacology > 1995 > 50 > 7 > 1007-1014
| journal ISSN : | 0006-2952 |
http://thirdworld.nl/a-simple-permanent-mount-of-sarcoptes-scabiei
Arch Dermatol
Authors:L.G. Arlian, R.A. Runyan, S. Achar, S.A. Estes1984
Authors:N.M. Nayel, M.T. Abu-Samra1986
Authors:Kate E. Mounsey, Hugh C. Murray, Helle Bielefeldt-Ohmann, Cielo Pasay, Deborah C. Holt, Bart J. Currie, Shelley F. Walton, James S. McCarthy2015
Authors:Xiaobin Gu, Yue Xie, Shuxian Wang, Xuerong Peng, Songjia Lai, Guangyou Yang2014
Authors:David P. Davis, Roger D. Moon1990
with SED who are already receiving this drug and (2) that PUVA therapy is used instead of systemic corticoste¬ roids in cases of SED as a new and better therapeutic alternative.
Karin Birgit Dahl, MD
Flemming Reymann, MD
Copenhagen 1. Andrews GC, Domonkos AN: Diseases of theSkin, ed 6. Philadelphia, WB Saunders Co, 1971, pp 221-224. 2. Rook A, Wilkinson DS, Ebling FJG: Textbook of Dermatology, ed 2. Oxford, Blackwell
Scientific Publications, 1972, vol 2, pp 278-281. 3. Rosenberg FR, Sanders S, Nelson CT:
Pemphigus: A 20-year review of 107 patients treated with corticosteroids. Arch Dermatol 112:962-970, 1976. 4. Parrish JA, Fitzpatrick TB, Tanenbaum L, et al: Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light. N
Engl J Med 291:1207-1211, 1974. 5. Walther JF, Vorhees JJ, Kelsey WH, et al:
Psoralen plus black light inhibits epidermal DNA synthesis. Arch Dermatol 107:861-865, 1973.
A Simple Permanent Mount of
Sarcoptes scabiei
To the Editor.\p=m-\In the current epidemic of scabies, more extensive efforts are being made to try to demonstrate the parasites. In some dermatological centers, at least in the past, it was required that to make a diagnosis of scabies the parasites had to be demonstrated. Most of the current techniques used for microscopic studies of skin scrapings are usually moist preparations. It is difficult to preserve such preparations for detailed search or for permanent preparations for records or for teaching purposes.
A simple technique for preserving the parasite is to select what you consider the primary fresh lesion. This
Permanent hair spray mount of Sarcoptes scabiei (May-Gr\l=u”\nwaldGiemsa stain, original magnification \m=x\200). is scraped gently on a clean slide and immediately sprayed with hair spray such as is done for permanent preparations of Papanicolaou smears. May\x=req-\
Gr\l=u”\nwaldGiemsa stain may be used after this to help identify the parasites and the ova especially when scanning under low power. A coverslip is applied and the preparation is now a permanent mount. This technique may also be used for canine scabies in dogs for permanent mounts for teach¬ ing and collection, although this para¬ site is easy to demonstrate in dogs.
This technique has probably been used before but is not well known.
Leon Goldman, MD
Cincinnati
Alopecia Areata and Vitiligo
Associated With Down’s Syndrome
To the Editor.\p=m-\Nineteencases of alopecia areata (AA) and four cases of vitiligo were noted in the skin of 214 patients with Down’s syndrome. Patients with Down’s syndrome are predisposed to immunological deficiency and thymus-dependent (T-cell) function. Drs Carter and Jegasothy suggested that immunological factors might contribute to the increased incidence of AA and vitiligo seen inpatients with Down’s syndrome.1 In the May Archives (113:688, 1977), a marked depression in peripheral blood
T-cell values of our patients with AA was compared with the values of 51 controls. The mean percentage of T\x=req-\ lymphocytes in AA was 58.9% compared with the control population of 74.9%. The number of T cells per cubic millimeter was 1,184 in patients with
AA compared with the normal control of 1,461/cu mm. The T-cell percentage and T-cell number in AA patients was significantly different from the controls (P < .001).
Ninety-seven percent of our patients with AA had depressed (< 70%)
T-cell levels in the peripheral blood.
The T-cell levels in the peripheral blood in 24 patients with vitíligo were determined by the same method because of the frequent association with AA.2 The patients ranged in age from 9 to 58 years, with a mean age of 35.9 years. The mean percentage of Tlymphocytes in patients with vitíligo was 55.2% compared with the control of 74.9%. The number of T cells per cubic millimeter in patients with vitíligo was 1,089.5 compared with the 1,460.7/cu mm value for the controls.
The T-cell percentage and T-cell num¬ ber in patients with vitíligo was significantly different from the con¬ trols (P < .05, and P < .001, respectively). Ninety-two percent of our patients (22/24) with vitíligo haddepressed (< 70%) T-cell values in the peripheral blood. Two of these pa¬ tients (11- and 18-year-old females) had Down’s syndrome, Hashimoto’s thyroiditis, and vitíligo. The 18-yearold patient with Down’s syndrome had vitíligo and AA. Her normal brother had AA. Sixteen of our vitíligo patients were female, and eight were male; there were three black patients.
There was no apparent relationship between age of the patient, and the number and the percent T cells. Dura¬ tion of disease did not alter our results. The observation by Carter and
Jegasothy1 of tinea pedis (76.6%) in
Down’s syndrome is notable, although the identification of the dermato¬ phyte would have been helpful. We have noted tinea corporis from Trichophyton rubrum in several patients with AA.3
Twenty-two of 24 vitíligo patients were tested for lymphocytotoxicity to our melanoma tumor cell line (M40) with an average percent toxicity of 80.8. The technique used was similar to that of Olkowski et al,4 except that melanin-containing tissue culture cells were used and were identified as
M40, melanoma tumor cell line. The lymphocytotoxicity results from 82 patients with AA against the same melanoma cell line (M40) averaged 71.2%. These percentages are not statistically different from one anoth¬ er.
We agree that the association of AA and vitíligo in Down’s syndrome lends support to the proposition that immu¬ nological factors contribute to the development of AA and vitiligo. Our studies also indicate a decrease in the number and percentage of T cells inpatients with vitiligo and AA. Lym¬ phocytotoxicity tests of 82 AA and 22 vitiligo patients against the same melanoma tumor cell line (M40) were not statistically different.
Algie C. Brown, MD
Zbigniew L. Olkowski, MD, ScD
John R. McLaren, MD
Mike H. Kutner, PhD
Atlanta 1. Carter DM, Jegasothy BV: Alopecia areata and Down syndrome. Arch Dermatol 112:1397\x=req-\ 1399, 1976. 2. Brown AC, Olkowski ZL, McLaren JR:
Zbigniew L. Olkowski, MD 